3509035
Promega T7 RNA Polymerase
DNA-dependent RNA polymerase for in vitro transcription.
Manufacturer: Fischer Scientific
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Color
Colorless
Purity
>90%
For Use With (Application)
Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage
-30°C to -10°C
Product Type
T7 RNA Polymerase
Form
Liquid
Components
5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
pH
7.7
Storage Buffer
20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source
Recombinant E. coli strain
Inhibitors
Recombinant RNasin™ Ribonuclease
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Description
- DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences >90% pure as determined by SDS polyacrylamide gel electrophoresis Free of detectable levels of contaminating RNase and DNase activity ( Incorporates 32P, 33P, 3H and 35S nucleoside triphosphates