3509032
Promega T4 DNA Ligase
For ligation of DNA ends.
Manufacturer: Fischer Scientific
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Color
Colorless
Purity
>90%
For Use With (Application)
Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Content And Storage
-30°C to -10°C
Product Type
T4 DNA Ligase
Form
Liquid
Components
10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
pH
7.4
Storage Buffer
10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source
RecombinantE. colistrain
Molecular Weight (g/mol)
68
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Description
- Joins Two DNA Strands with Cohesive or Blunt Ends Use with 5′, 3′ or blunt-ended dsDNA (e.g., inserts and vectors) Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule