3509040

Promega Exonuclease III

Exonuclease III is a 3' → 5' exonuclease specific for dsDNA.

Manufacturer: Fischer Scientific

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Concentration

150 to 200 U/μL

Components

10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2

pH

7.5

Storage Buffer

20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol

Source

Recombinant E. coli strain

Molecular Weight (g/mol)

28000

Form

Liquid

Color

Colorless

Purity

>90%

For Use With (Application)

Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA

Content And Storage

-30°C to -10°C

Product Type

Exonuclease III

Inhibitors

50mM NaCl

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3509032

For ligation of DNA ends....

Description

  • A 3′→5′ Exonuclease Specific for Double-Stranded DNA Control deletion rate by varying incubation temperature Inactivate Exonuclease III by heating at 75°C for 10 minutes Supplied at a concentration of 150-200u/μL