3509034
Promega T4 Polynucleotide Kinase
T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.
Manufacturer: Fischer Scientific
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Concentration
5 to 10 U/μL
Components
10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
pH
7.5
Storage Buffer
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Source
Recombinant E. coli strain
Molecular Weight (g/mol)
132
Form
Liquid
Color
Colorless
Purity
≥90%
For Use With (Application)
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Content And Storage
-30°C to -10°C
Product Type
T4 Polynucleotide Kinase
Inhibitors
Ammonium Ions and low levels of Phosphate Buffers
Related Products
Description
- Transfers the γ-Phosphate from ATP to the 5′ End of Nucleotides Can be inactivated by heating at 68°C for 10 minutes Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Labels the 5′ end of ssDNA, dsDNA and RNA molecules for use as probes or for sequencing or DNA-protein footprinting Supplied at a concentration of 5-10u/μL