3508996

Promega Alkaline Phosphatase, Calf Intestinal

Alkaline Phosphatase catalyzes the hydrolysis of 5'-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.

Manufacturer: Fischer Scientific

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Color

Colorless

pH

8

Storage Buffer

10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol

Source

Calf intestinal mucosa

Product Type

Alkaline Phosphatase

Components

10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine

For Use With (Application)

Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase

Content And Storage

-30°C to -10°C

Quantity

1000 U

Form

Liquid

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Description

  • Catalyzes the Hydrolysis of 5′ Phosphate Groups from DNA, RNA, and r- and dNTPs Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/μL Qualified for blue/white cloning; our assay provides a higher level of quality control for enzymes used in cloning applications