3509037
Promega Terminal Deoxynucleotidyl Transferase
TdT catalyzes the addition of mononucleotides to the terminal 3'-OH of a DNA initiator accompanied by the release of inorganic phosphate.
Manufacturer: Fischer Scientific
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Concentration
30 U/μL
Components
5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
For Use With (Application)
Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage
-30°C to -10°C
Product Type
Terminal Deoxynucleotidyl Transferase
Color
Colorless
pH
6.4
Storage Buffer
50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source
Recombinant E. coli strain
Form
Liquid
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Description
- Adds Mononucleotides to the Terminal 3′-OH of ssDNA and Releases PPi Adds mononucleotide tails to any type of 3′ end (3′ and 5′ overhangs or blunt ends) due to the presence of 1mM CoCl2 in the reaction buffer Quality tested for labeling of apoptotic DNA ends using the procedure outlined in the DeadEnd™ Fluorometric TUNEL System Technical Bulletin #TB235 for each lot of enzyme Supplied at a concentration of 30u/μL