BP81071
OPTIZYME™ DNase I (RNase-Free), Fisher BioReagents™
Manufacturer: Fischer Scientific
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Concentration
1 U/μL
pH
7.5
Storage Buffer
OPTIZYME™ DNase I Buffer 10X : Tris HCl 100mM (7.5 pH at 25°C), MgCl2 25mM, CaCl2 1mM, OPTIZYME™ EDTA 50mM (for inactivation by heat at 65°C for 10min.)
Source
E. coli cells with a cloned gene encoding bovine DNase I
Product Type
DNase I (RNase-free)
Color
Colorless
For Use With (Application)
Preparation of DNA-free RNA, removal of template DNA following in-vitro transcription, DNA labeling by nick translation in conjunction with DNA Polymerase I, Preparation of DNA-free RNA prior to RT-PCR, Studies of DNA-Protein interactions by DNase I, RNase-free footprinting
Content And Storage
-20°C
Quantity
1000 U
Form
Liquid
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Description
- OPTIZYME™ DNase I is a recombinant endonuclease that digests DNA and is RNase-Free
- Applications and Features: OPTIZYME DNase I, RNase-Free is used for the preparation of DNA-free RNA Removal of template DNA following in vitro transcription DNA labeling by nick translation in conjunction with DNA Polymerase I Preparation of DNA-free RNA prior to RT-PCR Studies of DNA-protein interactions by DNase I, RNase-free footprinting Enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+or Mn2+ions Purified from non-animal host with a lower level of intrinsic RNases Supplied with: 10X OPTIZYME DNase I Buffer: 100mM Tris-HCl (pH 7.5 at 25°C), 25mM MgCl 2 , 1mM CaCl 2 50mM OPTIZYME EDTA (for inactivation by heat at 65°C for 10min) Source: E
- Coli cells with a cloned gene encoding bovine DNase I Quality Control: Ribonuclease-free Functionally tested for the digestion of template DNA after in vitro transcription