K1034
CCF2-FA (free acid)
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1034-Each-of-1 | In Stock | ₹ 65,367.84 |
K1034 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 65,367.84
GST (18%): ₹ 11,766.211
Total Price: ₹ 77,134.051
Cell Permeability
Cell-Impermeant
Detection Method
Fluorescence
Product Line
GeneBLAzer™
Substrate
CCF2-FA
Substrate Type
Beta-Lactamase Substrate
Content And Storage
Store in freezer (-5 to -30°C).
Form
Powder
Quantity
100μg
Substrate Properties
Chemical Substrate
Shipping Condition
Approved for shipment on Wet or Dry Ice
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Description
- CCF2-FA (Free Acid) is a fluorescence resonance energy transfer (FRET) substrate that consists of a cephalosporin core linking B7-hydroxycoumarin to fluorescein
- This product is intended for use as a beta-lactamase substrate in a GeneBLAzer™ cell-based assay
- GeneBLAzer™ cell-based assays typically utilize the membrane-permeant ester forms (CCF2-AM and CCF4-AM) of the negatively charged fluorescent beta-lactamase substrates, CCF2 and CCF4
- These lipophilic esters readily enter the cell, where cleavage by endogeneous cytoplasmic esterases rapidly converts them into their negatively charged forms, thereby trapping them in the cytosol
- CCF2-FA is essentially the CCF2 substrate without the esters found in the AM version
- CCF2-FA is de-esterified and used in cell lysate applications, bypassing loading across the cell membrane and de-esterification steps
- Cell lysates are the preferred method for applications using cells that contain a cell wall
- CCF2-FA can also be used as a control to acquire the excitation and emission spectra for CCF2-AM and CCF4-AM.