Thermo Scientific™ IPTG

Description

• Thermo Scientific™ Pierce beta-Galactosidase Precipitating Substrates is a chromogenic substrate for beta-galactosidase that is used to detect expression of vectors, plasmids, or DNA fragments that contain the reporter gene
• X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate forβ-Galactosidase
• It yields a blue precipitate, is soluble in dimethylformamide, and can be used to detect vectors, plasmids or DNA fragments that contain the β-Galactosidase gene
• Highlights: See item-specific features X-Gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside) Chromogenic substrate for β-Galactosidase Yields a blue precipitate Soluble in DMF Can be used to detect vectors, plasmids, or DNA fragments that contain the β-Galactosidase gene IPTG (Isopropyl-β-D-thiogalactopyranoside) Maximizes the expression of genes in expression vectors When screening a lambda bacteriophage library with an antibody, the probability of success can be increased by preventing the expression of the fusion protein until the plaques are well established By placing a nitrocellulose filter containing the inducer IPTG onto the plate and continuing growth at 37°C, the expression of the potential β-galactosidase-cDNA fusion protein can be induced after three to four hours of plaque growth; the nitrocellulose filter is then screened with antibodies IPTG is also used in combination with X-Gal to detect active β-galactosidase Practical in applications using Bacteriophage M13 and Igt11 vectors