Invitrogen™ Rhodamine 110, bis-(p-Tosyl-L-Glycyl-L-Prolyl-L-Arginine Amide)

Description

• This Molecular Probes™ bisamide derivative of rhodamine 110 (R110) is a sensitive and selective substrate for assaying proteases in solution or inside living cells
• These fluorogenic substrates contain an amino acid or peptide covalently linked to each of R110's amino groups
• Upon enzymatic cleavage, the nonfluorescent bisamide substrate is converted first to the fluorescent monoamide and then to R110, with a further increase in fluorescence
• The substrate can be used to continuously measure enzyme activity in cell extracts and purified enzyme preparations using a fluorometer or fluorescence microplate reader, or can be used for intracellular protease assays with analysis by flow cytometry or fluorescence microscopy.< • Has spectral properties similar to those of fluorescein, making it compatible with standard fluorescein filter sets • Signal to background ratios are good because of large extinction coefficient of R110 • Unlike fluorescein, the fluorescence intensity of the rhodamine 110 reaction product is constant from pH 3–9 • Substrate name: p -tosyl-Gly-Pro-Arg) 2 -R110 • Useful to measure: Thrombin • Ex/Em of R110 product: (498/521nm) • Cell-Permeant Order Info Shipping Condition: Room temperature