Applied Biosystems™ PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit

Description

• The Applied Biosystems PrepSEQ 1-2-3 Nucleic Acid Extraction Kit rapidly extracts Mycoplasma genomic DNA from 100 μL sample volumes of research cell lines, stem cell lines, and cell and tissue therapy samples
• In the PrepSEQ 1-2-3 kit protocol, a rapid procedure recovers DNA from total lysate of the test sample using magnetic-bead based technology
• The PrepSEQ 1-2-3 kit is optimized for use with the MycoSEQ Mycoplasma Detection Kit
• Features of the PrepSEQ 1-2-3 Nucleic Acid Extraction Kit include: High recovery of Mycoplasma genomic DNA from mammalian cell culture samples Rapid easy-to-use protocol with results in under 2 hours Compatible with the MycoSEQ Mycoplasma Detection assay Protocol available for automated sample preparation High DNA recovery sample preparation Optimized for use with the MycoSEQ Mycoplasma Detection Kit, the PrepSEQ 1-2-3 Nucleic Acid Extraction Kit uses proprietary magnetic bead-based separation technology to extract Mycoplasma DNA from mammalian cell lines
• The PrepSEQ 1-2-3 kit extracts Mycoplasma genomic DNA from research cell lines, stem cell lines, and cellular and tissue therapy samples with high efficiency
• The protocol is recommended for use with a starting sample volume of 100 μL with a maximum cell density of 10 6 cells
• The PrepSEQ 1-2-3 kit provides an easy to use procedure consisting of 1) RNase treatment, 2) proteinase treatment, and 3) DNA extraction using the magnetic bead technology (figure below)
• The kit isolates Mycoplasma genomic DNA with high sensitivity from total lysate of test sample
• The PrepSEQ 1-2-3 kit and the MycoSEQ kit allow routine screening of high-value cell lines using an easy, rapid and sensitive protocol
• The figure below demonstrates how a sample that was spiked with low levels of Mycoplasma bovoculi DNA was found negative for Mycoplasma contamination using a commercially prepared kit
• When using the PrepSEQ 1-2-3 kit and the MycoSeq kit, the correct positive call for Mycoplasma contamination was made.