Cytiva plasmidPrep Midi Flow Kit
Manufacturer: Cytiva
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Description
illustra plasmidPrep Midi Flow Kit is designed for the purification of high yields of transfection-grade plasmid DNA. The kit allows for the purification of large yields of plasmid DNA that are proportional to the input sample volume.
Includes
Fast-flow genomic 250 columns, lyophilized Proteinase K powder, Lysis solutions, load and Elution Buffers, NAP-25 desalting column, spin column adapters, full protocol booklet, detachable, quick reference protocol card
Product Type
illustra plasmidPrep Midi Flow Kit
Sample Volume
150 mL Culture
For Use With (Application)
Used For the rapid extraction and purification of high-quality plasmid DNA from medium scale cultures for use in transfection, sequencing, and enzymatic amplification and modification
No. of Reactions
25 Preps
Purity
1.8
Test Time
Approx.215 min
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Description
- The kit consists of a high-performance, anion-exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA
- High column capacity allows for a proportional yield for input sample volume of 25 to 50mL (for high copy number plasmid) and up to 500mL for low copy number plasmid Provides plasmid DNA with excellent purity and low endotoxin levels Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns