Invitrogen™ Chargeswitch™ Pro Filter Plasmid Midi Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | CS31104-Each-of-1 | In Stock | ₹ 58,351.92 |
CS31104 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 58,351.92
GST (18%): ₹ 10,503.346
Total Price: ₹ 68,855.266
Column Type
Spin Column
Format
Spin Column
Sample Type
Bacterial Culture
Elution Volume
0.5 to 1 mL
For Use With (Application)
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Includes
Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer
Product Line
ChargeSwitch™
Quantity
25 preps
Shipping Condition
Room Temperature
System Type
ChargeSwitch™
Yield
200 μg
Content And Storage
The ChargeSwitch™-Pro Plasmid Kit contains Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
Isolation Technology
ChargeSwitch Chemistry
Scale
Midi
Final Product Type
Plasmid DNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Prep Scale
100-200 μg (Medium-Scale) Plasmid DNA
Product Type
Pro Filter Plasmid Midi Kit
Purity
Transfection Grade
Target
Plasmid DNA
Test Time
30 min.
Starting Material Amount
25 ml
Related Products
Description
- The ChargeSwitch™-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications
- The system is based upon a novel dual/nested column design which facilitate the fastest and simplest protocol for high quality plasmid
- The system is composed of two columns
- The inner flat bottomed column, also referred to as “lysate clarification” column, contains filter materials suitable for rapid clarification of bacterial lysate
- The outer luer bottomed column, also referred to as “binding” column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate
- This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step
- The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience
- 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method Cleaner purified product for improved performance in downstream applications A novel Dual/nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol Endotoxin units less than 10 EU/μg DNA for Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (Cat
- No
- K211101) How it works The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism
- At low pH values (less than 6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA
- Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid
- The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch™ method effortless
- Order Info Shipping Condition: Room Temperature
SAFETY INFORMATION
- ShelfLife : Guaranteed stable for 6 months when properly stored.