Thermo Scientific™ GeneJET Plasmid Midiprep Kit

Description

• Description GeneJET Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant E
• coli cultures
• The kit utilizes silica-based membrane technology in the form of convenient spin column
• Each prep recovers up to 200 μg of high copy number plasmid DNA
• The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines
• Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E
• coli cultures
• The kit utilizes silica-based membrane technology in the form of convenient spin column
• Each prep recovers up to 200 μg of high copy number plasmid DNA
• The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines
• Highlights • Efficient —high yields up to 200 μg of high quality plasmid DNA from 50 mL of bacterial culture • High concentration of DNA purified — >400 ng/μL • Convenient —spin column format, centrifuge or vacuum protocols • Fast —procedure takes 60 minutes, considerably faster than using gravity flow columns Applications Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including: • FastDigest restriction enzyme digestion • Automated fluorescent sequencing • PCR • Cloning • Transformation • In vitro transcription • Transfection of robust cell lines