MP116544050

MP Biomedicals™ SPINeasy™ DNA/RNA/Protein All-In-One Kit

Manufacturer: MP Biomedicals™

Select a Size

Pack Size SKU Availability Price
Each of 1 MP116544050-Each-of-1 In Stock ₹ 56,149.61

MP116544050 - Each of 1

₹ 56,149.61

In Stock

Quantity

1

Base Price: ₹ 56,149.61

GST (18%): ₹ 10,106.93

Total Price: ₹ 66,256.54

Content And Storage

Store at Room Temperature, Dry Environment

Sample Type

Blood, Cell Culture, Animal Tissue, Swabs

Quantity

50 Preps

Isolation Technology

Spin Column

Includes

50 x Lysing Matrix A, 60 mL Lysis Buffer R, 30 mL Wash Buffer D, 12 mL Wash Buffer R, 10 mL Elution Buffer GD, 10 mL Nuclease-free water, 1 vial DNase I, 5 mL DNase I Buffer, 80 mL Protein Precipitant, 100 x Column A with collection tube

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Description

  • SPINeasy DNA/RNA/Protein All-In-One Kit utilizes a convenient workflow and silica membrane spin columns to isolate DNA, RNA, and protein components from the same sample, without the use of toxic substances such as phenol and chloroform
  • The use of our specially formulated Lysis Buffer R and Lysing Matrix A in combination with FastPrep instruments from MP Biomedicals enables highly efficient lysis of tissue samples within seconds
  • DNA, RNA, and proteins are then sequentially purified from the same lysate
  • Briefly, DNA is absorbed onto the first spin column, while the flow-through is collected and used for RNA purification
  • The second spin column captures RNA and the flow-through from this step is used for protein extraction
  • DNA and RNA are bound on the first and second columns, respectively, and then washed and eluted
  • In the final extraction, proteins are precipitated out of solution, pelleted down by centrifugation, washed, and resuspended
  • Each molecular component is then immediately available for their respective downstream applications.