4925855
Invitrogen™ pBAD TOPO™ TA Expression Kit
Specifically designed for one-step cloning and regulated prokaryotic expression ofTaq-amplified PCR products
Manufacturer: Fischer Scientific
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Constitutive or Inducible System
Inducible
Promoter
araBAD
Selection Agent (Eukaryotic)
None
Bacterial or Yeast Strain
LMG194, TOP10
Expression System
E. coli
Cloning Method
TOPO-TA
Product Line
TOPO™
Inducing Agent
Arabinose
Product Type
TA Expression Kit
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Cleavage
EK (Enterokinase) Recognition Site
Expression Mechanism
Cell-Based Expression
Vector
pBAD
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Description
- Linearized, topoisomerase I-activated vector for 5-minute cloning of Taq amplified PCR products araBAD promoter for tightly regulated expression in E
- coli V5 epitope tag for detection with Anti-V5 Antibody C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody Enterokinase cleavage site for removal of N-terminal leader peptide Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis