V43001

Invitrogen™ pBAD/His Kit

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 V43001-Each-of-1 In Stock ₹ 94,287.12

V43001 - Each of 1

₹ 94,287.12

In Stock

Quantity

1

Base Price: ₹ 94,287.12

GST (18%): ₹ 16,971.682

Total Price: ₹ 1,11,258.802

Constitutive or Inducible System

Inducible

Promoter

araBAD

Selection Agent (Eukaryotic)

None

Antibiotic Resistance Bacterial

Ampicillin (AmpR)

Protein Tag

His Tag (6x)

Quantity

1 kit

Inducing Agent

Arabinose

Product Type

Bacterial Expression Vector

Content And Storage

The pBAD/His Kit includes 20 μg of each pBAD/His A, B, & C vector, 20 μg of pBAD/His/lacZ, 1 ml of 20% L-arabinose, LMG194 and TOP10E. colistabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.

Cleavage

EK (Enterokinase) Recognition Site

Cloning Method

Restriction Enzyme/MCS

Vector

pBAD

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Description

  • araBAD promoter for tightly regulated expression Translation initiation signals optimized for E
  • coli expression N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with Anti-HisG Antibody N-terminal Xpress epitope for detection and analysis with Anti-Xpress Antibody Enterokinase cleavage site for removing the N-terminal tag following purification Three vectors are provided (A, B, and C): Each has the N-terminal tag in a different reading frame relative to multiple cloning site to simplify in-frame cloning of gene Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis