Thermo Scientific™ pJTI™ R4 Dest CMV N-EmGFP pA Vector

Description

• The pJTI™ R4 Dest CMV N-EmGFP pA vector allows a gene of interest to be expressed as an N-terminal Emerald Green Fluorescent Protein (EmGFP) fusion in a Jump-In™ parental cell line
• When used in combination with a Jump-In™ parental cell line kit such as the Jump-In™ GripTite™ HEK293 cell line (Cat
• No
• A14150), an isogeneic stable cell line can be created with less effort and in less time than traditional cell engineering methods
• In addition, Gateway™ technology increases the efficiency of the cloning steps to introduce your GOI into the Gateway™-ready pJTI™ R4 Dest CMV N-EmGFP pA vector
• The high retargeting efficiency, made possible by the R4 sites in the Jump-In™ parental cell line, allows the use of the isogenic pool for additional experiments without the need for clonal selection
• Alternatively, the high retargeting efficiency allows for easy selection of a positive stable clone for expressing your gene of interest with an N-terminal EmGFP
• The Jump-In™ Vectors and Parental Cell Lines Let You: Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods
• Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms or orthologs
• Generate multiple cell lines in parallel due to the simplified work flow
• Easily access the Jump-In™ technology with the ability to generate an unlimited number of cell lines without complicated licenses or restrictions to interpret
• Save Time with Rapid and Efficient Generation of Engineered Cell Lines Using Gateway™ technology, you can generate an expression construct for retargeting using the pJTI R4 DEST CMV N-EmGFP pA destination vector
• In combination with a Jump-In™ parental cell line kit, the expression cassette is efficiently and specifically inserted into a genomic target R4 site resulting in the generation of functional cell pools in as little as 2 weeks without laborious clone isolation and analysis
• Even generation of clonal cell lines can be done with reduced time and effort due to the high percentage of positive clones
• Expand Your Experimental Capabilities The pJTI™ R4 Dest CMV N-EmGFP pA vector along with a Jump-In™ parental cell line provide the ideal solution for cells and assays where transient engineering technologies are problematic, as well as for difficult to 'engineer' cell lines
• The kit also provides a convenient way to create target panels of gene families, isoforms, or orthologs
• Genes coding for large proteins or multi-unit proteins are not a problem since the Gateway™ destination vectors accept large inserts.