A15005
pJTI™ R4 EXP CMV-TO EmGFP pA Vector
Manufacturer: LIFE TECHNOLOGIES
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A15005-Each-of-1 | In Stock | ₹ 25,496.88 |
A15005 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 25,496.88
GST (18%): ₹ 4,589.438
Total Price: ₹ 30,086.318
Constitutive or Inducible System
Inducible
Inducing Agent
Tetracycline
Product Type
Mammalian Expression Vector
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Quantity
100 μg
Product Line
Jump-In™
Delivery Type
Transfection
Promoter
CMV/TO
Reporter Gene
GFP (EmGFP)
Content And Storage
pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 μg), store at -20 °C
Protein Tag
GFP (EmGFP)
Vector
Jump-In Vectors
For Use With (Application)
Stable Cell Line Development, Targeted Integration, Regulated Expression
Related Products
Description
- The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line
- When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection, the EmGFP can be inducibly expressed with doxycycline and the successfully retargeted cells will fluoresce green
- Ensure the Success of Your Jump-In™ T-REx™ Retargeting Reactions Successful retargeting of Jump-In™ T-REx™ parental cell lines like the Jump-In™ T-REx™ HEK293 Kit is dependent on a variety of factors, such as: Transfection efficiency Cell confluency Antibiotic selection conditions Quality and concentration of DNA Retargeting vector to integrase vector ratio We strongly recommend including a positive control retargeting reaction using the pJTI™ R4 Exp CMV-TO EmGFP pA vector in your Jump-In™ experiment, along with negative controls (no plasmid DNA, no integrase vector), so you can easily visualize the results and optimize the retargeting conditions.