K481001

Invitrogen™ NT-GFP Fusion TOPO™ Expression Kit

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 K481001-Each-of-1 In Stock ₹ 1,02,843.12

K481001 - Each of 1

₹ 1,02,843.12

In Stock

Quantity

1

Base Price: ₹ 1,02,843.12

GST (18%): ₹ 18,511.762

Total Price: ₹ 1,21,354.882

Constitutive or Inducible System

Constitutive

Promoter

CMV

Reporter Gene

GFP (Cycle 3)

Content And Storage

GFP Fusion TOPO™ Expression Kits contain two boxes. The GFP Fusion TOPO™ TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. The One Shot ¤ box contains transformation reagents including single-use, 50-μl aliquots of One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and supercoiled control plasmid. Store the One Shot™ box at -80°C. Kits are guaranteed stable for 6 months when properly stored.

Cloning Method

TOPO-TA

Vector

TOPO-TA Vectors

For Use With (Application)

Reporter Assays

Delivery Type

Transfection

Product Type

TOPO Expression Kit

Selection Agent (Eukaryotic)

Geneticin™ (G-418)

Protein Tag

GFP (Cycle 3)

Quantity

20 reactions

Product Line

TOPO™

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Description

  • Provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO vector Taq -amplified DNA fragments are ligated into these vectors in easy 5-minute reaction on bench top Vector is designed for high-level expression of transient or stable GFP fusion proteins in wide range of mammalian cells Expression is easily detected in living cells using fluorescence Recombinant proteins expressed from these vector can be detected on western blots using GFP Antiserum Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Constitutive Expression, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g
  • GFP) Assays, Fluorescent Protein Assays (GFP)/Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays