K35920

Invitrogen™ Vivid Colors™ pcDNA™6.2/C-EmGFP-GW/TOPO™ Mammalian Expression Vector

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 K35920-Each-of-1 In Stock ₹ 2,07,483.00

K35920 - Each of 1

₹ 2,07,483.00

In Stock

Quantity

1

Base Price: ₹ 2,07,483.00

GST (18%): ₹ 37,346.94

Total Price: ₹ 2,44,829.94

Constitutive or Inducible System

Constitutive

Promoter

CMV

Reporter Gene

GFP (EmGFP)

Content And Storage

Each pcDNA™6.2-TOPO™ Expression Vector Kit contains two boxes. The TOPO™ box contains linearized and topoisomerase I-activated pcDNA™6.2-TOPO™ vector, control template, primers for sequencing, PCR reaction components, and an expression control plasmid. The TOPO™ box should be stored at -20°C. The One Shot™ box contains transformation reagents including single-use 50 μl aliquots of One Shot™ TOP10 chemically competentE. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store competentE. coliat -80°C. All reagents are guaranteed stable for 6 months when properly stored.

Cloning Method

TOPO-TA

Vector

pcDNA

For Use With (Application)

Reporter Assays, Subcellular Localization

Delivery Type

Transfection

Product Type

Mammalian Expression Vector

Selection Agent (Eukaryotic)

Blasticidin

Protein Tag

GFP (EmGFP)

Quantity

20 reactions

Product Line

Gateway™, TOPO™, Vivid Colors™, pcDNA™

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Description

  • Powerful TOPO cloning vectors contain Emerald Green Fluorescent Protein (EmGFP) or Yellow Fluorescent Protein (YFP) for simple, noninvasive detection of recombinant protein Both FPs have been humanized for optimal mammalian expression Topoisomerase I for one-step, 5-minute TOPO cloning of PCR-amplified gene of interest CMV promoter for high-level expression of recombinant fluorescent fusion protein Ability to fuse EmGFP or YFP to N- or C-terminus of protein Bsd resistance marker for rapid selection of stable cell lines Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Cloning, Constitutive Expression, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g
  • GFP) Assays, Fluorescent Protein Assays (GFP)/Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays