PQ1000101
Invitrogen™ ProQuest™ Two-Hybrid System with Gateway™ Technology
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PQ1000101-Each-of-1 | In Stock | ₹ 1,41,002.88 |
PQ1000101 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,41,002.88
GST (18%): ₹ 25,380.518
Total Price: ₹ 1,66,383.398
Product Type
Two-Hybrid System
Content And Storage
The ProQuest™ Two-Hybrid System includes yeast expression vectors pDEST™32, pDEST™22, and pEXP-AD502 for generation of GAL4 DNA Binding Domain and GAL4 Activation Domain fusion proteins, and LR Clonase™ II Enzyme Mix, a glycerol stock of the MaV203 yeast strain, as well as positive and negative controls for the two-hybrid assay. Store kit components at -80°C. Guaranteed stable for six months when properly stored.
Expression System
Yeast
Product Line
Gateway™, ProQuest™
Reporter Gene
Beta-Gal (lacZ), HIS3, URA3
Assay
Two-Hybrid Assay
Quantity
1 kit
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Description
- Reconstitutes a functional transcription factor that activates chromosomally integrated reporter genes where transcriptional activation is monitored by the growth of cells on selective media Allow rapid and easy generation of bait and prey constructs, and facilitate downstream application Low copy-number DBD (DNA Binding Domain) and AD (Activation Domain) vectors ( ARS/CEN ) to control over-expression and increase reproducibility Three different reporter genes ( HIS 3, URA 3, and lac Z) with independent promoter regions to rapidly weed out false positives URA 3 reporter gene allows for both positive and negative selection, enabling advanced two-hybrid techniques such as reverse two-hybrid A set of strong-to-weak Gateway-based control interactions to evaluate results and assess the strength of the interaction being tested Protein-Protein Interactions, Proteins, Expression, Isolation and Analysis, Yeast Two-Hybrid