V35120

Invitrogen™ pRSET A, B, & C Bacterial Expression Vectors

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 V35120-Each-of-1 In Stock ₹ 91,720.32

V35120 - Each of 1

₹ 91,720.32

In Stock

Quantity

1

Base Price: ₹ 91,720.32

GST (18%): ₹ 16,509.658

Total Price: ₹ 1,08,229.978

Constitutive or Inducible System

Inducible

Promoter

T7

Selection Agent (Eukaryotic)

None

Antibiotic Resistance Bacterial

Ampicillin (AmpR)

Protein Tag

His Tag (6x)

Quantity

20 μg

Inducing Agent

IPTG

Product Type

Bacterial Expression Vector

Content And Storage

20 μg of each pRSET A, B, & C vector is provided lyophilized. TOP10F´, BL21(DE3)pLysS, and BL21 pRSET/lacZ control E. coli stabs are also included. Store E. coli stabs at 2–8°C. All components are guaranteed stable for 6 months when properly stored.

Cleavage

EK (Enterokinase) Recognition Site

Cloning Method

Restriction Enzyme/MCS

Vector

pRSET

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Description

  • Expression is induced by production of T7 RNA polymerase in BL21(DE3) E
  • coli These cells also produce T7 lysozyme to reduce basal expression of target genes pRSET vector offers bacteriophage T7 promoter for high-level expression T7 gene 10 sequence to provide protein stability N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody N-terminal Xpress epitope for detection with the Anti-Xpress Antibody Enterokinase cleavage site for removal of fusion tag Each of three vectors provided (A, B, and C) has N-terminal tag coding sequence in a different reading frame relative to multiple cloning site to simplify in-frame cloning of the gene Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis