V53220

Invitrogen™ pBudCE4.1 Mammalian Expression Vector

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 V53220-Each-of-1 In Stock ₹ 78,030.72

V53220 - Each of 1

₹ 78,030.72

In Stock

Quantity

1

Base Price: ₹ 78,030.72

GST (18%): ₹ 14,045.53

Total Price: ₹ 92,076.25

Constitutive or Inducible System

Constitutive

Promoter

EF-1α, CMV

Selection Agent (Eukaryotic)

Zeocin™

Protein Tag

His Tag (6x), V5 Epitope Tag, c-Myc Epitope Tag

Quantity

20 μg

For Use With (Application)

Multi-Gene Expression

Delivery Type

Transfection

Product Type

Mammalian Expression Vector

Content And Storage

20 μg each of pBudCE4.1 and pBudCE4.1/lacZ/CAT positive control vectors are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

Cloning Method

Restriction Enzyme/MCS

Vector

pBud

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Description

  • Using pBudCE4.1 to generate stable mammalian expression cell lines ensures that there is an equivalent copy number of each gene in cell; this can eliminate variable expression due to differences in gene copy number Provides expression cassettes with the CMV promoter for high-level transcription of genes with optional c-myc epitope tag for rapid detection and 6xHis sequence for simple purification Human EF-1α promoter for high-level expression of genes with optional V5 epitope tag for rapid detection and 6xHis sequence for simple purification Sh ble (ZeoR) gene for efficient selection in both mammalian cells and E
  • coli with selection agent Zeocin™ Constitutive Expression, Mammalian Expression, Multi-Gene Expression, Protein Expression, Proteins, Expression, Isolation and Analysis