V90020

Invitrogen™ pSecTag2 A, B, & C Mammalian Expression Vectors

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 V90020-Each-of-1 In Stock ₹ 1,03,356.48

V90020 - Each of 1

₹ 1,03,356.48

In Stock

Quantity

1

Base Price: ₹ 1,03,356.48

GST (18%): ₹ 18,604.166

Total Price: ₹ 1,21,960.646

Delivery Type

Transfection

Product Type

Mammalian Expression Vector

Content And Storage

20 μg of each vector and a PSA expression control are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

Cloning Method

Restriction Enzyme/MCS

Vector

pSec

Promoter

CMV

Selection Agent (Eukaryotic)

Zeocin™

Protein Tag

His Tag (6x), c-Myc Epitope Tag, IgK Leader Sequence

Quantity

20 μg

For Use With (Application)

Secreted Expression

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Description

  • Each vector has large multiple cloning site in three reading frames to simplify cloning in frame with N-terminal secretion signal Secretion signal from the V-J2-C region of mouse Ig kappa-chain for efficient secretion of recombinant proteins Cytomegalovirus (CMV) promoter for high-level constitutive expression C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with Anti-His(C-term) Antibody C-terminal c- myc epitope for detection with Anti- myc Antibody Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen ( e.g., COS-1, COS-7)T pSecTag2 vectors carry Zeocin resistance gene for cost-effective selection in mammalian cells
  • Zeocin selection can also be used in E
  • coli Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Secreted Expression