Thermo Scientific™ Biotin Chromogenic Detection Kit

Description

• Thermo Scientific Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes
• The kit is optimized to reproducibly provide high sensitivity with a low background in applications, such as Southern, Northern, dot and slot blotting, and screening of viral plaques and bacterial colonies
• Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling
• Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods, including the Biotin DecaLabel DNA Labeling Kit (Cat
• No
• K0651)
• Features Biotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP)
• Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes
• The probes are then visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate
• Visualization does not require X-ray film or other specific equipment ( see Figure 1)
• Highlights • High sensitivity of detection —0.03 pg of homologous DNA in dot blot hybridization with biotin-labeled probe • Low background due to optimized washing and blocking procedures • Non-radioactive detection visualization does not require X-ray film or other specific equipment ( see Figure 2) • Fast and convenient —ready-to-use components Applications • Southern, Northern, dot/slot blots, plaque, or colony screening
• Note: This kit is optimized for hybridization procedures using nylon membranes
• The detection sensitivity may vary depending on the membranes supplied by different vendors.