Thermo Scientific™ LightShift™ Chemiluminescent EMSA Kit

Description

• Thermo Scientific™ LightShift Chemiluminescent EMSA Kit is an extraordinarily robust and sensitive system for performing electrophoretic mobility shift assays (EMSA) to identify and characterize protein-DNA binding interactions
• The kit includes reagents for setting up and customizing protein:DNA binding reactions, a control set of DNA and protein extract to test the kit system, stabilized streptavidin-HRP conjugate to probe for the biotin-labeled DNA target, and an exceptionally sensitive chemiluminescent substrate module for detection.The principle for LightShift™ EMSA Detection is similar to a Western blot
• Biotin end-labeled duplex DNA is incubated with a nuclear extract or purified factor and electrophoresed on a native gel
• The DNA is then rapidly (30 minutes) transferred to a positive nylon membrane, UV-crosslinked, probed with streptavidin-HRP conjugate and incubated with the substrate
• The protocol from labeling to results can be accomplished in a single day
• All you need to perform the assay are purified DNA target that has been end-labeled with biotin, the protein extract you wish to test, nylon membrane and basic electrophoresis equipment
• DNA targets may be synthesized with 5' or 3' biotin labels or they may be labeled after synthesis using the Thermo Scientific Biotin 3' End DNA Labeling Kit (Part No
• 89818)
• Nuclear, cytosolic or whole cell protein extracts may be obtained by a variety of methods, including the Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit (Part No
• 78833)
• Highlights: Includes EBNA control system to help new users develop a working assay and understand the methods used to confirm binding interaction specificity Excellent for detecting low-abundance proteins in nuclear extracts Sensitivity that surpasses radioactive and digoxigenin methods Compatible with previously established binding conditions for popular DNA:protein interactions