4918729
Invitrogen™ mRNA Catcher™ PLUS Purification Kit
The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format.
Manufacturer: Fischer Scientific
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Isolation Technology
Oligo dT Affinity
Elution Volume
80 μL
Final Product Type
mRNA
For Use With (Equipment)
Robotic Liquid Handling Systems
Shipping Condition
Room Temperature
Yield
>80 ng
Purification Time
1.5 hr. (Including sample homogenization)
Sample Type
Cells, Tissue, Total RNA, Whole Blood
High-throughput Compatibility
High-throughput Compatible
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
Starting Material Amount
Cells: 100–106Tissue: 4–40 mgTotal RNA: 0.1–100 μgWhole Blood: 40 μL
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Description
- The mRNA Catcher™ PLUS Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format
- By taking advantage of Locked Nucleic Acid (LNA) technology, the mRNA Catcher PLUS Kit provides improved performance and a faster protocol, while preserving mRNA yield and purity
- How it works mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA
- The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding
- The mRNA can either be eluted or directly reversed-transcribed to cDNA directly in the well
- The mRNA Catcher PLUS method does not require centrifugation, precipitation, phase separation, or column filtration
- In addition, the entire method is amenable to automation for streamlined, high-throughput expression profiling for large-scale processes
- Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR
- • Automated protocols, high-throughput compatible • Robust performance: high binding capacity per well (>80ng/well) • Reproducible results: low CVs from well-to-well and plate-to-plate (∽ 2%) • Optimized protocols: for small sample sizes (100 cells, 4 mg tissue, and 40 μL blood) • Compatible plate design: may be used with most PCR cyclers Order Info Shipping Condition: Wet ice