Invitrogen™ RiboPure™ RNA Purification Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1924-Each-of-1 | In Stock | ₹ 54,587.28 |
AM1924 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 54,587.28
GST (18%): ₹ 9,825.71
Total Price: ₹ 64,412.99
Content And Storage
• 50 mL TRI Reagent; store at 4°C• 50 Filter Cartridges; room temperature• 100 Collection Tubes (2 mL); room temperature• 60 mL Wash Solution (concentrated); room temperature• 11 mL Elution Buffer; room temperature
Purification Time
30 min. (After sample homogenization)
Sample Type
Cells, Tissue
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
50 Preps
Starting Material Amount
Cells: 0.1–20 x 106Tissue: 5–100 mg
Isolation Technology
Spin Column (Glass Fiber Filter)
Elution Volume
100 μL
Final Product Type
Total RNA
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Shipping Condition
Room Temperature
Yield
Up to 500 μg
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Description
- The Ambion™ RiboPure™ Kit is designed for rapid purification of high-quality RNA from tissue samples or cultured cells
- The kit combines TRI Reagent™ with glass fiber filter purification to yield exceptionally pure RNA, free of residual proteins and lipids
- The kit includes sufficient reagents for 50 purifications from 1–100 mg of tissue or 0.1–20 x 10 6 cultured cells each
- • Yields the highest purity RNA attainable • Ideal for the most stringent downstream applications • Handles problematic samples with ease • Fast (less than 30 minutes) and easy procedure RNA isolated using the RiboPure™ Kit is suitable for use in all common downstream applications, including cDNA synthesis, real-time and end-point RT-PCR, microarray analysis, northern blots, and RNase protection assays
- The RiboPure™ protocol is especially suited for processing samples that are difficult to disrupt, or samples that have large amounts of ribonuclease or other contaminants
- Samples are first homogenized in TRI Reagent™, a monophasic solution containing phenol and guanidine thiocyanate, to rapidly lyse cells and inactivate nucleases
- Addition of bromochloropropane (BCP) results in separation of the homogenate into aqueous and organic phases
- RNA partitions to the aqueous phase, while DNA and protein remain in the interphase and organic phase
- The aqueous phase is removed and passed through a glass fiber filter that binds the RNA
- The filter is washed, and the RNA is eluted
- The procedure is compatible with tissues that have been stored in Ambion's RNA later ™
- Total RNA yield is typically 100–500 μg per 100 mg of tissue, depending on the type of tissue
- Order Info Shipping Condition: Room temperature