Applied Biosystems™ MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit

Description

• The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit is designed to recover RNA and DNA from virus and gram-negative bacteria in samples such as blood, swabs, urine, and viral transport media (VTM)
• The kit utilizes MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid compatible with a broad range of applications, including real-time PCR, digital PCR, and next-generation sequencing
• Features of the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit include: • Automation-ready protocols designed for the KingFisher Flex and KingFisher Duo Prime systems • Fast procedure allows for 96 samples to be processed in under 30 minutes • Flexible protocol accommodates sample volume inputs from 200 μL to 2 mL of biofluids or transfer media • No need for carrier RNA • Elution volumes ranging from 50 to 100 μL Maximum sample input in a 96-deep well or 24-deep well plate format The optimized reagents included in the MagMAX Viral/Pathogen kit allow you to maximize the amount of sample input, thereby increasing the amount of RNA and/or DNA recovered
• Up to 400 μL of sample can be processed per well in a KingFisher 96-deep well plate
• For larger sample volumes, protocols are available to process up to 2 mL of sample per well in a KingFisher 24-deep well plate
• Benefits of MagMAX magnetic bead–based purification Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid
• Beads bind the nucleic acid more efficiently than glass-fiber filters, resulting in higher and more consistent yields
• Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples
• This clogging issue is of particular concern with protein-rich, large-volume samples such as whole blood or plasma that are commonly used for viral testing
• The Dynabeads MyOne Silane contained in the MagMAX Viral/Pathogen Total Nucleic Acid Isolation Kit have an optimized silica-like surface chemistry with a highly specific surface area that allows efficient kinetics and high sensitivity in nucleic acid capture
• These aspects make Dynabeads MyOne Silane ideal for the recovery viral RNA and DNA present at low concentrations in body fluids
• Once captured, these nucleic acids can then be eluted in 50 μL of elution buffer for use in downstream applications.