A1083708
Ambion™ RiboMinus™ Eukaryote Kit for RNA-Seq
Manufacturer: Ambion™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A1083708-Each-of-1 | In Stock | ₹ 76,148.40 |
A1083708 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 76,148.40
GST (18%): ₹ 13,706.712
Total Price: ₹ 89,855.112
Content And Storage
Each kit contains:• 6 mL RiboMinus™ Magnetic Beads• 80 μL RiboMinus™ Eukaryote Probe (15 pmol/μL)• 10 mL Hybridization Buffer• 12 mL DEPC-treated (RNase-free) waterStore at 4°C.
Sample Type
Total RNA
High-throughput Compatibility
High-throughput Compatible
No. of Reactions
8 Preps
Quantity
1 Each
Shipping Condition
Wet Ice
Target
Transcriptome RNA
Yield
1 μg
Isolation Technology
Magnetic Bead
Final Product Type
Transcriptome RNA
For Use With (Application)
Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
Product Line
RiboMinus™
Sequencing Type
Transcriptome Sequencing
Starting Material Amount
2-10 μg Total RNA
System Type
RiboMinus
Related Products
Description
- The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome
- The RiboMinus™ Eukaryote Kit for RNA-Seq Offers: • A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA • Rapid and efficient removal of rRNA-probe complex using magnetic bead technology Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure
- Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit
- Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module (Cat
- No
- K155005).