Invitrogen™ Poly(A)Purist™ MAG Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1922-Each-of-1 | In Stock | ₹ 82,479.84 |
AM1922 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 82,479.84
GST (18%): ₹ 14,846.371
Total Price: ₹ 97,326.211
Content And Storage
Store Nuclease-free Water, Microfuge Tubes and Microfuge tubes with Spin Columns at room temperature. Store 2X Binding Solution, Wash Solution 1, Wash Solution 2 and Oligo(dT) Cellulose at 4°C. Store RNA Storage Solution, 5M Ammonium Acetate and Glycogen(5 mg/mL) at -20°C.
Isolation Technology
Magnetic Bead, Oligo dT Affinity
Final Product Type
mRNA
For Use With (Application)
Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), Microinjection, Nucleic Acid Labeling, In Vitro Translation, cDNA Library Construction, Northern Blotting, Nuclease Protection Assays
Product Type
MAG Kit
Target
mRNA
Format
Kit
Sample Type
Total RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Product Line
Ambion™, Poly(A)Purist™
Shipping Condition
Room Temperature
Test Time
45 min.
Related Products
Description
- Ambion™ Poly(A)Purist™ MAG Kit (patent pending) uses magnetic technology for the selection of mRNA that typically requires only a single round of selection yet yields RNA that is more highly enriched and devoid of rRNA than other procedures
- Each kit contains sufficient reagents for the isolation of mRNA from up to 8 mg of total RNA
- • Highest recovery of mRNA of any available kit • High stringency for lowering residual rRNA content • Single round of selection sufficient for most applications The superior performance of Poly(A)Purist™ MAG Kit is clearly demonstrated by a comparison of the mRNA recovered using 4 other kits (see Figure)
- The kit uses batch binding of RNA to premeasured aliquots of oligo(dT) cellulose to circumvent the problems of slow flow rates and clogged columns often experienced during conventional, gravity-driven chromatography
- The magnetic bead–based selection employed in the delivers increased processing speed, ease of handling, and scalability
- The entire selection procedure can be completed in as little as 45 minutes (see Figure)
- Accessory Products: A magnetic stand is required for use this kit
- They are available in several formats, including the Single Place Magnetic Stand (Cat
- No
- AM10026) and the 6 Tube Magnetic Stand (Cat
- No
- AM10055)
- User Guide Alert (July 2023): Step 3 of the Preparation of Oligo (dT) MagBeads section (page 7) of the User Guide is misleading
- Please follow this instruction for Step 3: add an equivalent mass of Oligo (dT) Magbeads into the selected empty tube and then continue with Oligo (dT) Magbeads preparation according to steps 4-9
- Order Info Shipping Condition: Room Temperature