18-080-400
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-080-400-Each-of-1 | In Stock | ₹ 55,442.88 |
18-080-400 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 55,442.88
GST (18%): ₹ 9,979.718
Total Price: ₹ 65,422.598
Content And Storage
• 2X Reaction Mix, 500 μL• SuperScript™ III Enzyme Mix, 100 μL• Oligo(dT)20, 50 μL total (50 μM)• Random hexamers, 50 μL (50 ng/μL)• Annealing buffer, 50 μLStore at –20°C.
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Primer
Random Primers, Oligo dT Primers
Shipping Condition
Dry Ice
Final Product Type
First-Strand cDNA
Reaction Format
Master Mix
Starting Material
RNA
Format
Kit
Reaction Speed
50 min.
Optimal Reaction Temperature
50°C
Reverse Transcriptase
SuperScript III
For Use With (Application)
Real Time PCR (qPCR)
No. of Reactions
50 Reactions
Reagent Type
Reverse Transcription
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Description
- The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A) + or total RNA
- RNA targets from 100 bp to >12 kb can be detected with this system
- The amount of starting material can vary from 0.1 pg to 5 μg of total RNA
- This kit includes SuperScript III/RNaseOUT enzyme mix, 2X First-Strand reaction mix, and an annealing buffer
- SuperScript III Reverse Transcriptase is a version of MMLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability
- The enzyme can be used to synthesize cDNA at a temperature range of 45–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than many previous reverse transcriptases
- Using SuperScript III First-Strand Synthesis SuperMix SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA and can be used to synthesize cDNA from total RNA
- RNaseOUT Recombinant RNase Inhibitor is included in the enzyme mix to safeguard against degradation of target RNA due to ribonuclease contamination
- The 2X First-Strand reaction mix includes 10 mM MgCl 2 and 1 mM of each dNTP in a buffer formulation that has been optimized for first-strand synthesis of cDNA
- The annealing buffer is used in the initial template-primer annealing step
- Separate tubes of oligo(dT) 20 and random hexamers are also provided
- cDNA synthesis can be performed using either total RNA or poly(A) + -selected RNA primed with oligo(dT), random primers, or a gene-specific primer
- Order Info Shipping Condition: Dry ice