Thermo Scientific™ Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq

Description

• Thermo Scientific Verso 1-Step RT-PCR Kit ReddyMix with ThermoPrime Taq is a complete set of RT-PCR reagents that combines everything needed for RT-PCR
• The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures
• Verso RT also shows a better dynamic range than standard AMV or M-MuLV enzymes, with increases in template concentration giving rise to corresponding increases in product generated over a wider range of starting material concentrations
• An optional RT enhancer is provided with the kit and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment
• This Verso 1-step RT-PCR system comes with the ReddyMix option, where the master mix contains a dye and precipitant that allow RT-PCR reactions to be loaded directly onto an electrophoresis gel
• The red dye migrates between bromophenol blue and xylene cyanol at approximately 300 bp, depending on agarose concentration
• Features of the Verso 1-Step RT-PCR Kit ReddyMix with ThermoPrime Taq include: • ReddyMix formulation—eliminates post-PCR steps required for electrophoresis • Eliminates the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over • RT and PCR steps performed in single sealed tube
• providing convenience and reduced contamination risk • Verso RT enzyme is highly sensitive with a broad dynamic range—accurately detect RNA input from 1 pg to 1 μg • Wide working temperature range (42–57°C) for success with GC-rich and other difficult templates • RNase Inhibitor included in RT enzyme mix Applications • Gene expression analysis • Viral/bacterial detection • 1-step RT-PCR • High throughput applications Related products Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq (Cat
• No
• AB1454LDA) Verso 1-Step RT-PCR Kit, with Thermo-Start Taq (Hot Start) (Cat
• No
• AB1455A)