43-444-63

Applied Biosystems™ SYBR™ Green Universal Master Mix

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 43-444-63-Each-of-1 In Stock ₹ 13,518.48

43-444-63 - Each of 1

₹ 13,518.48

In Stock

Quantity

1

Base Price: ₹ 13,518.48

GST (18%): ₹ 2,433.326

Total Price: ₹ 15,951.806

Content And Storage

SYBR™ Green PCR Master Mix in a 1 mL vial. Sufficient reagents provided for 40 reactions based on a 50 μl reaction volume. Store at -20°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Form

Concentrated

GC-Rich PCR Performance

High

Polymerase

AmpliTaq Gold DNA Polymerase

Technique

Fluorescence Intensity

Label or Dye

SYBR Green

Product Line

SYBR™

Quantity

1 x 1 mL

Sufficient For

40 Reactions of 50 μL

For Use With (Application)

Gene Expression

Hot Start

Built-In Hot Start

Sample Type

DNA

Detection Method

SYBR

Format

Tube

PCR Method

qPCR

Reaction Speed

Standard

For Use With (Equipment)

7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, AB 7900 HT 384-Well System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, GeneAmp™ 5700

Passive Reference Dye

ROX (Pre-mixed)

Product Type

Real Time PCR SYBR Master Mix

Shipping Condition

Wet Ice

Target Specificity

Not Target-Specific

Concentration

2X

No. of Reactions

40 Reactions

Volume

1 x 1 mL

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Description

  • Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial
  • SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase Proprietary buffer enhancements ensure performance and reliability Premixed components stored at 2° to 8°C significantly reduce assay setup time Maximum Flexibility and Convenience: Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed
  • Store at -20°C Order Info Shipping Condition: Wet Ice