Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Description

• The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction
• Both types of kit deliver extremely high-quality, single-stranded cDNA from 0.02–2 μg total RNA
• Reactions can be scaled up to 100 μL to generate 10 μg of cDNA from a single reaction
• The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor includes the recombinant enzyme used to prevent degradation of RNA templates and lacks DNA endonuclease activity for better product yield
• In addition to their use in RT-PCR, this kit may be used in traditional PCR, microarrays, and for the generation of cDNA archives
• Features of the High Capacity cDNA Reverse Transcription Kit include: • Linear target amplification for real-time PCR • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost • 10-fold greater dynamic range than other kits Extensively tested with a variety of templates Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers
• This kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels
• cRNA can also be efficiently generated from in vitro transcription of cDNA
• Order Info Shipping Condition: Dry ice