43-889-50
Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 43-889-50-Each-of-1 | In Stock | ₹ 2,65,663.80 |
43-889-50 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,65,663.80
GST (18%): ₹ 47,819.484
Total Price: ₹ 3,13,483.284
Content And Storage
• 20X Enzyme mix (1 x 500 μL)• 2X RT Buffer Mix (5 x 1 mL)Store at –15 to –25°C.2X RT Buffer Mix can be stored at 2–8°C for up to 6 months.
Reaction Speed
30 to 60 min.
Optimal Reaction Temperature
37°C
Ribonuclease H Activity
Yes
For Use With (Application)
Real Time PCR (qPCR), RT-PCR
No. of Reactions
500 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Format
Kit
Technique
Reverse Transcription
Reverse Transcriptase
MultiScribe
Shipping Condition
Dry Ice
Final Product Type
First-Strand cDNA
Reaction Format
Premixed Components
Size (Final Product)
Up to 7 kb
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Description
- Description The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts
- Features of the High Capacity RNA-to-cDNA Kit include: • Fast reaction time (typically 30–60 min) • Convenient and simple workflow with few pipetting steps • Reliable reverse transcription of both abundant and limited targets • Optimized two-step protocol enables multiple PCR reactions from a single RT reaction Streamline cDNA synthesis while maintaining detection sensitivity Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude
- This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes
- The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.