Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Description

• Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR
• Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu , Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity
• Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases
• Features of Phusion™ High-Fidelity DNA Polymerase include: High fidelity (52X Taq ) Fast PCR due to short extension times (15–30 s/kb) Robust performance, minimal optimization needed High yields of PCR products with minimal enzyme amounts Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L) Applications High-fidelity PCR Cloning Template generation for sequencing Amplification of difficult (GC-rich) templates Long-range PCR (up to 20 kb) Mutagenesis High throughput PCR Microarray Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases)
• For optimal results, use our convenient Tm calculator.