4527036
Thermo Scientific™ Maxima SYBR Green/ROX qPCR Master Mix (2X)
Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.
Manufacturer: Fischer Scientific
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Detection Method
SYBR
GC-Rich PCR Performance
High
Polymerase
Taq DNA Polymerase
For Use With (Equipment)
7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Product Type
Real Time PCR SYBR Master Mix
Concentration
2X
Format
Tube
PCR Method
qPCR
Reaction Speed
Standard
Product Line
Maxima
For Use With (Application)
Gene Expression
Sample Type
DNA, dsDNA
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Description
- The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer
- Only template and primers need to be added
- The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes
- Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity
- dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG)
- The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates
- Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers
- Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers Sensitivity - detects low copy number targets Wide linear range - accurate quantification across 9 orders of magnitude Reproducibility and convenience - ready-to-use 2X master mix