Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, ROX

Description

• The Thermo Scientific Verso 1-step RT-qPCR ROX Kit for SYBR Green chemistry offers a quick and simple way to detect mRNA
• An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment
• All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof
• In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR)
• Adopting a 1-step approach is ideal for primer sequence optimization and collecting comparative data in experiments that do not require subsequent cDNA storage
• The approach minimizes the risk of contamination, and improvements in reproducibility may be observed as a result of reduced sample handling
• Verso One-Step RT-qPCR Kits for SYBR Green chemistry are compatible with all major real-time thermal cyclers
• Eliminate the need for DNase treatments - RT Enhancer (optional) prevents gDNA carry-over RT and qPCR steps performed in single tube - convenience and reduced contamination risk Use of the entire RT reaction as template for qPCR - improved sensitivity Verso RT enzyme is highly sensitive with broad dynamic range - accurate detection of RNA from 1pg to 1μg Verso RT enzyme has a wide working temperature range (42° to 57°C) - improved success with GC-rich and other difficult templates Contains an inert blue dye for ease of pipetting during reaction setup