7181282
Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
Manufacturer: Fischer Scientific
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Description
Works on all AB platforms, but is optimized for the 7500.
Format
Tube
PCR Method
1-step RT-qPCR
Reaction Speed
Standard
Passive Reference Dye
ROX (Pre-mixed)
Product Type
One-Step RT-PCR Reagent
Concentration
25X
Detection Method
Primer-probe
GC-Rich PCR Performance
High
Polymerase
AmpliTaq Gold DNA Polymerase
For Use With (Equipment)
7500 System
Product Line
AgPath-ID™, Ambion™
Reverse Transcriptase
ArrayScript™
Fidelity (vs. Taq)
25 X
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Description
- Recommended for RNA pathogen amplification Consistent amplification of RNA targets with high specificity and sensitivity Optimized to work with your target-specific primers and probes Contains ROX for quantitative fluorescent signal normalization AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup
- The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time
- Once assembled, results are available in approximately one hour
- The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification
- The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.