Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)

Description

• Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR
• Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu , Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity
• Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase
• With Phusion Hot Start II High-Fidelity DNA Polymerase amplification proceeds without the production of nonspecific products due to the combination of Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand that inhibits DNA polymerase activity at room temperature
• The Affibody ligand also inhibits the 3' to 5' exonuclease activity of the polymerase, thus preventing degradation of primers and template DNA during reaction set up
• At temperatures that promote polymerase activity, the ligand is released, rendering the polymerase fully active
• Phusion Hot Start II DNA Polymerase is immediately reactivated at high temperatures, so it does not require a separate activation step in PCR protocols
• Phusion Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion Hot Start II DNA Polymerase and the 5X Phusion buffers
• The buffers include a density reagent and two tracking dyes which do not interfere with the robust performance of Phusion Hot Start II DNA Polymerase
• Researchers may directly load PCR products on a gel making this a versatile option for use in downstream applications such as DNA sequencing, ligation and restriction digestion
• Highlights Reaction set up at room temperature No non-specific amplification and primer degradation during reaction set up Zero-time reactivation due to unique hot start technology High fidelity (52X Taq) Fast PCR due to short extension times (15–30 s/kb) Robust reactions, minimal optimization needed Increased product yields with minimal enzyme amounts Direct loading on gels Applications High-fidelity PCR High throughput Amplification of difficult (GC-rich) templates Template generation for sequencing Multiplex PCR Long-range PCR Cloning Mutagenesis Microarray Using Phusion DNA polymerases Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).