Thermo Scientific™ Phusion U Hot Start DNA Polymerases

Description

• Thermo Scientific Phusion U DNA Polymerase is a novel engineered high fidelity enzyme developed using fusion technology
• Due to a proprietary mutation in the so called dUTP binding pocket of Phusion polymerase, Phusion U polymerase overcomes an important limitation of proofreading enzymes: it is able to incorporate dUTP and read through uracil present in DNA templates
• In addition to the uracil usage possibility, Phusion U polymerase features all the superior properties of other Phusion DNA polymerases: great accuracy, speed, ability to amplify long amplicons up to 20 kb, and a high specificity with Affibody-based hot start
• These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control
• Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green buffers
• The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel
• The colored buffer does not interfere with Phusion U polymerase performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion
• Highlights Accuracy —high fidelity DNA polymerase (25X Taq) Uracil-tolerance —engineered to incorporate dUTP and amplify uracil containing templates Specificity —hot start for reduced nonspecific amplification and primer degradation Speed —short extension times (15–30 s/kb) Green format —permits direct loading of PCR products on gels Applications Amplification of bisulphite-converted DNA Amplification of damaged or aged DNA Carry-over contamination control Uracil-excision based (USER) cloning methods Using Phusion DNA polymerases Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).