12-033-015
Invitrogen™ ElectroMAX™ DH10B T1 Phage-Resistant Competent Cells
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 12-033-015-Each-of-1 | In Stock | ₹ 31,999.44 |
12-033-015 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 31,999.44
GST (18%): ₹ 5,759.899
Total Price: ₹ 37,759.339
Product Type
Electrocompetent Cells
Improves Plasmid Quality
Yes (endA1)
Transformation Efficiency Level
High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial
Yes (Streptomycin)
Blue/White Screening
Yes (lacZΔM15)
Plasmid
May be used for plasmids >20 kb
Reduces Recombination
Yes (recA1)
T1 Phage - Resistant (tonA)
Yes
Format
Tube
Quantity
5 x 100 μL
Contains F' Episome
No
Cloning Methylated DNA
Yes (mcrA)
Content And Storage
• ElectroMAX DH10B T1 Phage-Resistant Cells (5 x 100 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. Medium (2 x 6 mL)Store S.O.C. Medium at 4°C or room temperature..
Cloning Unstable DNA
Not suitable for cloning unstable DNA
High-throughput Compatibility
Low
Preparing Unmethylated DNA
No
Shipping Condition
Dry Ice
Species
E. coli (K12)
Product Line
ElectroMAX™
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Description
- ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1 x 10 10 cfu/μg plasmid DNA in efficiency
- They are ideal for applications requiring high-efficiency transformation, including cDNA and gDNA library construction
- ElectroMAX DH10B cells: • Maximize yield of transformants from limited cloning products • Permit efficient cloning of methylated DNA • Are resistant to T1 and T5 bacteriophages • Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal • Deliver high-yield plasmid preparations for downstream applications Phage-resistant representative libraries from a single transformation The ton A mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable libraries against contamination
- Additionally, mutations in the methylation-dependent restriction system ( mcr A, mcr BC, and mrr ) make ElectroMAX DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA and allow for efficient plasmid rescue from eukaryotic genomes
- ElectroMAX DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for situations when DNA is limiting
- This strain also has the Φ80 lac ZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal
- Finally, the end A1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification
- Genotype F - mcr A Δ( mrr - hsd RMS- mcr BC) Φ80 lac ZΔM15 Δ lac X74 rec A1 end A1 ara D139Δ( ara , leu )7697 gal U gal K λ - rps L nup G ton A Find the strain and format that fits your needs • DH10B cells are available in both electrocompetent and chemically competent formats
- • ElectroMAX DH10B cells (Cat
- No.18290015) are suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection
- • MegaX DH10B T1 R Electrocomp Cells (Cat
- No
- C640003) have the highest transformation efficiency (>3 x 10 10 cfu/μg plasmid DNA) and also offer the benefit of T1 and T5 phage resistance
- Order Info Shipping Condition: Dry Ice