18-290-015
Invitrogen™ ElectroMAX™ DH10B Cells
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-290-015-Each-of-1 | In Stock | ₹ 31,314.96 |
18-290-015 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 31,314.96
GST (18%): ₹ 5,636.693
Total Price: ₹ 36,951.653
Product Type
Electrocompetent Cells
Improves Plasmid Quality
Yes (endA1)
Transformation Efficiency Level
High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial
Yes (Streptomycin)
Blue/White Screening
Yes (lacZΔM15)
Plasmid
May be used for plasmids >20 kb
Reduces Recombination
Yes (recA1)
T1 Phage - Resistant (tonA)
No
Format
Tube
Quantity
5 x 100 μL
Contains F' Episome
No
Cloning Methylated DNA
Yes (mcrA)
Content And Storage
• ElectroMAX DH10B Cells (5 x 100 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. medium (2 x 6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA
Not suitable for cloning unstable DNA
High-throughput Compatibility
Low
Preparing Unmethylated DNA
No
Shipping Condition
Dry Ice
Species
E. coli (K12)
Product Line
ElectroMAX™
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Description
- ElectroMAX DH10B Cells are electrocompetent E
- coli cells offering the highest transformation efficiencies of >1 x 10 10 cfu/μg plasmid DNA
- The high transformation efficiencies make these cells ideal for cloning both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes
- ElectroMAX DH10B Cells are suitable for construction of gene banks, generation of cDNA libraries using plasmid-derrived vectors, and for situations when DNA is limited
- They are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction
- ElectroMAX DH10B Cells provide: • High-efficiency transformation to maximize clones • Enhanced genetic markers for cDNA or gDNA cloning capabilities ElectroMAX DH10B Cell features • Φ80 lac ZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal • mcr A genotypic marker and mcr BC, mrr deletion that allow cloning DNA that contains methylcytosine and methyladenine • end A1 mutation that enables increased plasmid yield and quantity Genotype F - mcr A Δ( mrr - hsd RMS- mcr BC) Φ80 lac ZΔM15 Δ lac X74 rec A1 end A1 ara D139Δ( ara , leu )7697 gal U gal K λ - rps L nup G Get more detailed information about specific Genotypes and Genetic Markers of our E
- coli Competent Cells
- Find the strain and format that you need DH10B cells are available in both electrocompetent and chemically competent formats
- ElectroMax DH10B T1R Cells (Cat
- No
- 12033015) and MegaX DH10B T1 R Electrocomp Cells (Cat
- No
- C640003) offer the benefit of T1 and T5 phage resistance
- Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required
- Order Info Shipping Condition: Dry Ice