12-034-013
Invitrogen™ MAX Efficiency™ DH5α-T1RCompetent Cells
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 12-034-013-Each-of-1 | In Stock | ₹ 14,545.20 |
12-034-013 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 14,545.20
GST (18%): ₹ 2,618.136
Total Price: ₹ 17,163.336
Product Type
Chemically Competent Cells
Improves Plasmid Quality
Yes (endA1)
Transformation Efficiency Level
High Efficiency (>1 x 109cfu/μg)
Antibiotic Resistance Bacterial
No
Blue/White Screening
Yes (lacZΔM15)
Preparing Unmethylated DNA
No
Shipping Condition
Dry Ice
Species
E. coli (K12)
Product Line
MAX Efficiency™
Contains F' Episome
No
Cloning Methylated DNA
No
Content And Storage
• MAX Efficiency DH5α-T1RCompentent Cells (5 x 200 μL)Store Competent Cells at –80°C.• pUC19 DNA (50 μL at 10 pg/μL)Store pUC19 DNA at –20°C.• S.O.C. Medium (2 x 6 mL)Store S.O.C. Medium at 4°C or room temperature.
Cloning Unstable DNA
Not suitable for cloning unstable DNA
High-throughput Compatibility
Low
Reduces Recombination
Yes (recA1)
T1 Phage - Resistant (tonA)
Yes
Format
Tube
Quantity
5 x 200 μL
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Description
- MAX Efficiency DH5α T1 R Phage-Resistant Competent Cells are high efficiency chemically competent cells for generating cDNA libraries and were developed to confer resistance to the lytic bacteriophages T1 and T5
- In addition to supporting blue/white screening, rec A1 and end A1 mutations in DH5α increase insert stability and improve the quality of plasmid DNA prepared from minipreps
- These cells are suitable for the construction of gene banks or generation of cDNA libraries using derived vectors
- MAX Efficiency DH5α T1 R Phage-Resistant Competent Cells have been prepared by a patented modification of the procedure of Hanahan and yield >1 x 10 9 transformants/μg of monomer pUC19 DNA in a 100 μL transformation reaction
- MAX Efficiency DH5α T1 R Phage Resistant Competent Cell features • Resistance to the lytic bacteriophage T1, one of the most dangerous E
- coli phages in high throughput laboratories and genomic centers • The φ80 lac ZΔM15 marker that provides α-complementation of the ß-galactosidase gene from pUC or similar vectors and, therefore, can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal • Capable of being transformed efficiently with large plasmids and can serve as a host for the M13mp cloning vectors if a lawn of DH5α-FT, DH5αF', DH5αF'IQ, JM101, or JM107 is provided to allow plaque formation Genotype F - φ80 lac ZΔM15 Δ(lacZYA- arg F)U169 rec A1 end A1 hsd R17(rk-, mk+) pho A sup E44 λ- thi -1 gyr A96 rel A1 ton A Order Info Shipping Condition: Dry Ice