Invitrogen™ Click-IT™ L-Homopropargylglycine (HPG)

Description

• Click-iT homopropargylglycine (HPG) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional technique, 35S methionine, for the detection of nascent protein synthesis
• HPG is an amino acid analog that contains a very small modification, specifically an alkyne moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis
• Detection utilizes the chemoselective ligation or “click” reaction between and azide and an alkyne where the alkyne modified protein is detected with an azide-containing dye or hapten together with either the Click-iT Cell Reaction Buffer Kit or the Click-iT Protein Buffer Kit
• With the Click-iT Cell Reaction Buffer Kit, cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis
• With the Click-iT Protein Reaction Buffer Kit, achieve detection sensitivity in 1-D gels and western blots in the low femtomole range or perform LC-MS/MS and MALDI MS analysis
• Less hazardous Cell Analysis, Click Chemistries, Glycoprotein Analysis with Click Chemistry, Labeling Chemistry, Metabolic Labeling and Nascent Protein Synthesis with Click Chemistry Order Info Shipping Condition: Room temperature