Invitrogen™ Click-IT™ Palmitic Acid, Azide (15-Azidopentadecanoic Acid)

Description

• Identify and characterize palmitylated proteins with Click-iT palmitic acid, azide using the powerful click chemistry, a simple and robust two-step labeling and detection technique
• In step one, the azide-containing biomolecule is fed to cells or animals and actively incorporated into proteins
• Unlike other labels such as biotin or a fluorescent dye, the azide-tag is small enough that the tagged molecule is an acceptable substrate for the enzymes that incorporate this building block into proteins
• Detection utilizes the chemoselective ligation or “click” reaction between and azide and an alkyne where the modified protein is detected with the corresponding alkyne-containing dye or hapten using either the Click-iT Cell Reaction Buffer Kit or the Click-iT Protein Buffer Kit
• With the Click-iT Cell Reaction Buffer Kit, cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis (HCS) together with other biomarkers of interest for content and context rich results
• With the Click-iT Protein Reaction Buffer Kit, achieve detection sensitivity in 1-D gels and western blots in the low femtomole range or perform LC-MS/MS and MALDI MS analysis
• Less hazardous Cell Analysis, Click Chemistries, Labeling Chemistry, Metabolic Labeling and Nascent Protein Synthesis with Click Chemistry Order Info Shipping Condition: Room temperature