Thermo Scientific™ Pierce™ SAT(PEG)4 (PEGylated N-succinimidyl S-acetylthioacetate)

Description

• Thermo Scientific™ Pierce SAT(PEG) 4 is a long-arm (18.2 Ås), pegylated form of SATA, a sulfhydryl-addition reagent for covalent modification of primary amines to facilitate controlled, heterobifunctional crosslinking
• SAT(PEG) 4 extends the flexibility, solubility and reach of other S-acetylthio (SAT) reagents for sulfhydryl modification of amine-containing molecules
• SAT(PEG) 4 has the same form as SATA and SATP, which are widely used as part of protein conjugation strategies
• Two purified proteins (e.g., antibody and enzyme) are conjugated after modifying one protein with an SAT reagent and the other protein with a maleimide-activating crosslinker such as Sulfo-SMCC (Part No
• 22322)
• Because SAT reagents add a protected sulfhydryl, the modified protein can be stored indefinitely before treating it to expose the sulfhydryl and performing the final sulfhydryl-directed conjugation reaction.The four-unit polyethylene glycol (PEG) group in SAT(PEG) 4 provides increased hydrophilicity and spacer-arm length relative to SATA and SATP
• When used in combination with a long-arm PEG-containing crosslinker like SM(PEG) 12 (Part No
• 22112), SAT(PEG) 4 can be used to crosslink proteins with long spacers
• Highlights: Reacts with primary amines to add protected sulfhydryls An easy deprotection step generates a free sulfhydryl group Water-soluble polyethylene glycol (PEG) spacer arm enhances solubility of labeled protein