Thermo Scientific™ EZ-Link™ Alkoxyamine-PEG4-Biotin

Description

• Thermo Scientific™ EZ-Link Alkoxyamine-PEG4-Biotin is a biotinylation reagent containing a four-unit polyethylene glycol (PEG) spacer for biotinylating macromolecules at carbohydrate groups that have been oxidized to form aldehydes
• Alkoxyamine-activated biotinylation reagents such as this one are useful for biotin-labeling glycoproteins and other molecules that have oxidizable polysaccharides groups
• EZ-Link Alkoxyamine-PEG-Biotin reagents contain a multi-functional extended spacer arm that is a flexible, non-immunogenic hydrophilic polyethylene glycol (PEG), which imparts water solubility to labeled molecules
• Consequently, antibodies labeled with biotin alkoxyamine reagents exhibit less aggregation when stored in solution compared to antibodies labeled with reagents having only hydrocarbon spacers
• Highlights: Biotin – label macromolecules for detection or affinity purification with avidin and streptavidin Alkoxyamine-activated – reacts with aldehyde groups (e.g., oxidized sugars in glycoproteins) to attach biotin by stable oxime bonds Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and improve the solubility of labeled antibodies Spacer arm – the reach between the point of attachment and the biotin affinity tag is nearly 27 angstroms, sufficient to minimizing steric hindrance for binding Specifications: Chemical formula: C 18 H 34 N 4 O 6 S Molecular weight: 434.55 Spacer arm: 27.0 angstroms Net mass added to target: 416.53 Form: white solid Solubility: dissolve in DMSO to make 250mM stock